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Irina Polejaeva

Profile Picture

ADVS

Developmental Biology

Associate Professor

Educational Background

Other, Stem Cell Biology, Utah State University, 1996
PhD, Developmental Biology, (Area of specialization: Stem cell biology), National Research Institute of Animal Science, 1993
Establishment, genetical transformation and the use of embryonic stem cell lines.
MS, Animal Science, Kubanski Agricultural Institute, 1985
Efficiency using meprin in ration of hens


Biography

Dr. Polejaeva joined the ADVS department in April 2011 as an Associate Professor. She is a member of the Veterinary Diagnostics and Infectious Disease USTAR team and Utah Collaborative Arrhythmia Program.


Research Interests

The area of her research interest includes: • Production of transgenic animals using somatic cell nuclear transfer technology (cloning) for biomedical and agricultural applications; • Improve efficiency of Somatic Cell Nuclear Transfer (SCNT) through understanding molecular mechanism of epigenetic reprogramming; • Development of accurate pre-implantation embryo assessment methods using combination of individual embryo culture system, imaging and gene expression/metabolomic analysis


Contact Information

Office Location: AGRS 244
DialPhone: 435-797-3718
SendEmail: irina.polejaeva@usu.edu

Awards

Best of Basic Science, 2013

American Heart Association Scientific Sessions

  • Thomas, A., Jones, R., Regouski, M., Polejaeva, I., Davies, C., (2016). Identification of Transgene Insertion Sites by Target Selection and Next-Generation Sequencing. Proceedings of the Large Animal Genetics Engineering Summit
  • Polejaeva, I., Ranjan, R., Davies, C., White, K.L, (2016). TGF-β1 Transgenic Goat Model of Atrial Fibrillation. Proceedings of the Large Animal Genetics Engineering Summit
  • Hu, S., Hall, J., Wang, Z., Polejaeva, I., (2014). TALEN-mediated targeted insertion of transforming growth factor-β1 (TGF-β1) gene into the goat AAVS1 locus. . Transgenic research
  • Polejaeva, I., Ranjan, R., Wang, Z., Olsen, A.L, Davies, C., White, K.L, (2014). Transgenic goat models to study cardiac fibrosis and atrial fibrillation. . Transgenic research
  • Hall, J., Yang, M., Meng, Q., Dai, J., Polejaeva, I., (2014). Effect of follicular size of cytoplast donor on cloning efficiency in goats.. Reproduction, Fertility and Development
  • Hu, S., Wang, Z., Polejaeva, I., (2014). Knockout of goat nucleoporin 155 (NUP155) gene using CRISPR/CAS9 systems.. Reproduction, Fertility and Development
  • Polejaeva, I., Ranjan, R., Hall, J., Rutigliano, H., Thomas, A.J, Dosdall, D., MacLeod, R., Marrouche, N., Wang, Z., Olsen, A.L, White, K.L, Davies, C., (2013). Cardiac specific overexpression of Transforming Growth Factor Beta1 (TGF-Beta1) increases susceptibility to atrial fibrillation in transgenic goats. Circulation
  • Rutigliano, H., Wilhelm, A., Hall, J., Sessions, B., Meng, Q., White, K.L, Bunch, T.D, Davies, C., Polejaeva, I., (2013). Aberrant gene expression at the fetal-maternal interface in sheep and goats produced by somatic cell nuclear transfer. Amernican Journal of Reproductive Immunology
  • Hall, J., Meng, Q., Sessions, B.R, Fan, Z., Wang, X., Stott, R.D, Rutigliano, H., Davies, C., Panter, K., Bunch, T.D, White, K.L, Polejaeva, I., (2012). Effect of embryo culture length on production of cloned transgenic goats. Reproduction, Fertility & Develpment
  • Meng, Q., Hall, J., Rutigliano, H., Zhou, X., Sessions, B.R, Stott, R.D, Panter, K., Davies, C., Ranjan, R., Dosdall, D., MacLeod, R., Marrouche, N., White, K.L, Wang, Z., Polejaeva, I., (2012). Generation of cloned transgenic goats with cardiac specific overexpression of transforming growth factor β1. Reproduction, Fertility & Develpment
  • Polejaeva, I., Hall, J., Meng, Q., Zhou, X., Sessions, B.R, Panter, K., Stott, R.D, Rutigliano, H.M, Davies, C., Wang, Z., Ranjan, R., Dosdall, D., MacLeod, R., Marouche, N., White, K.L, (2012). Development of a transgenic goat model with cardiac-specific overexpression of human transforming growth factor-β1 to study the relationship between atrial fibrosis and atrial fibrillation. Circulation Research
  • Tessanne, K., Stroud, T., Long, C., Sadeghieh, S., Hwang, E., Chen, S., Polejaeva, I., Westhusin, M., (2009). Development of transgenic livestock with reduced myostatin expression using RNA interference. Reproduction, Fertility and Development
  • Xiang, T., Walker, S., Gregg, K., Zhou, W., Farrar, V., Sadeghieh, S., Hwang, W., Findeisen, B., Arenivas, F., Polejaeva, I., (2009). Reprogramming of Oct-4 following equine somatic cell nuclear transfer. Reproduction, Fertility and Development
  • Gregg, K., Chen, S., Sadeghieh, S., Guerra, T., Xiang, T., Meredith, J., Polejaeva, I., (2009). Risk assessment of infectious disease transmission via in vitro embryo production using somatic cell nucleus transfer. Reproduction, Fertility and Development
  • Ayaers, D., Kind, A., Schnieke, A., Campbell, K., McCreath, K., Howcroft, J., Emslie, L., Mycock, K., Gibson, Y., Dai, Y., Polejaeva, I., Phelps, C., Geyer, C., Vaught, T., Mullins, J., Coleman, A., (1999). Gene targeting in livestock. Transgenic research
  • Polejaeva, I., Ashktorab, H., Bunch, T., Ellis, L., Reed, W., White, K., (1996). Stable transfection of mink embryonic stem (ES) cells and the expression of green fluorescent protein under cytomegalovirus promoter control. Theriogenology
  • White, K., Polejaeva, I., Bunch, T., Suh, T., Reed, W., Spendlove, R., Wilkinson, R., (1995). Effect of non-serum supplemented media on establishment and maintenance of bovine embryonic stem-like cells. Theriogenology
  • Polejaeva, I., White, K., Ellis, L., Reed, W., (1995). Isolation and long-term culture of mink and bovine embryonic stem-like cells. Theriogenology

Publications - Books & Book Chapters

    Book Chapters

  • Polejaeva, I., (2006). A double nuclear transfer technique for cloning pigs. : Methods of Molecular Biology. Methods of Molecular Biology.

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Publications - Fact Sheets

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    Publications - Curriculum

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      Publications - Journal Articles

        Academic Journal

      • Yun, S., Song, B., Polejaeva, I.A, Davies, C.J, White, K.L, Lee, Y., (2016). Comparison of the live-attenuated Japanese encephalitis vaccine SA14-14-2 strain with its pre-attenuated virulent parent SA14 strain: similarities and differences in vitro and in vivo. Journal of General Virology (J Gen Virol), 97:10, 2575-2591. doi: 10.1099/jgv.0.000574
      • Polejaeva, I., Ranjan, R., Davies, C., Regouski, M., Hall, J., Olsen, A.L, Meng, Q., Rutigliano, H., Dosdall, D., Angel, N., Sachse, F., Seidel, T., Thomas, A., Stott, R.D, Panter, K., Lee, P., Van Wettere, A., Stevens, J.R, Wang, Z., MacLeod, R., Marrouche, N., White, K.L, (2016). Increased Susceptibility to Atrial Fibrillation Secondary to Atrial Fibrosis in Transgenic Goats Expressing Transforming Growth Factor-β1. Journal of Cardiovascular Electrophysiology. , 27:10, 1220-1229.
      • Yun, S., Song, B., Frank, J.C, Julander, J.G, Polejaeva, I.A, Davies, C.J, White, K.L, Lee, Y., (2016). Complete genome sequences of three historically important, spatiotemporally distinct, and genetically divergent strains of Zika virus: MR-766, P6-740, and PRVABC-59. Genome Announcements (Genome Announc), 4:4, e00800-16. doi: 10.1128/genomeA.00800-16
      • Yun, S., Song, B., Frank, J.C, Julander, J.G, Polejaeva, I.A, Davies, C.J, White, K.L, Lee, Y., (2016). Complete genome sequences of three historically important, spatiotemporally distinct, and genetically divergent strains of Zika virus: MR-766, P6-740, and PRVABC-59. Genome Announcements (Genome Announc), 4:4, e00800-16. doi: 10.1128/genomeA.00800-16
      • Martins, L.T, Polejaeva, I., N., , , , , , , , , , (2016). Developmental outcome and related abnormalities in goats: comparison between somatic cell nuclear transfer- and in vivo-derived concepti during pregnancy through term.. Cellular reprogramming, 18:4, 264-279. doi: 10.1089/cell.2015.0082
      • Wang, M., Hall, j., Fan, Z., Regouski, M., Meng, Q., Rutigliano, H., Stott, R.D, Rood, K., Panter, K., Polejaeva, I., Oocytes from small and large follicles exhibit similar development competence following goat cloning despite their differences in meiotic and cytoplasmic maturation. Theriogenology
      • Polejaeva, I., Rutigliano, H., Wells, K.D, (2016). Livestock in biomedical research: history, current status and future prospective. Reproduction, Fertility and Development, 28:2, 112-124.
      • Rutigliano, H., Wilhelm, A., Hall, J., Shi, B., Meng, Q., Stott, R.D, Bunch, T.D, White, K.L, Davies, C., Polejaeva, I., (2015). Cytokine gene expression at the maternal-fetal interface after somatic cell nuclear transfer pregnancies in small ruminants.. Reproduction, Fertility, and Development, doi: doi:10.1071/RD15103
      • Rutigliano, H., Wilhelm, A., Hall, J., Shi, B., Meng, Q., Stott, R., Bunch, T.D, White, K.L, Davies, C., Polejaeva, I., Cytokine gene expression at the maternal?fetal interface after somatic cell nuclear transfer pregnancies in small ruminants.. Reproduction, Fertility and Development
      • Ni, W., Qiao, J., Hu, S., Zhao, X., Regouski, M., Yang, M., Polejaeva, I., Chen, C., (2014). Efficient gene knockout in goats using CRISPR/Cas9 system.. PLoS One, 2014, 9 (9), :e106718
      • Gong, J., Chen, G., Wang, Z., Polejaeva, I., Silisga, R., Chen, C., Kao, C., Chen, L., (2014). Activation of human K-ras G12D stimulates oncogenic transformation in transgenic goat fetal fibroblast cells. . PLoS One, 9(3): e90059
      • Zhou, W., Gosch, ., Guerra, T., Broek, D., Wu, G., Walker, S., Polejaeva, I., (2014). Amino acid profiles in first trimester amniotic fluids of healthy cloned pregnancies are similar to those of IVF pregnancies, but not nonviable cloned pregnancies . Theriogenology, 81:2, 225-229. doi: http://dx.doi.org/10.1016/j.theriogenology.2013.09.012
      • Polejaeva, I., Broek, D., Walker, S., Zhou, W., Walton, M., Benninghoff, A., Faber, D., (2013). Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. PLoS One, 8:12, e84283. doi: 10.1371/journal.pone.0084283
      • Polejaeva, I., Mitalipov, S., (2013). Stem cell potency and the ability to contribute to chimeric organisms. Reproduction , 145:3, R81-88. doi: 10.1530/REP-12-0396
      • Mendicino, M., Ramsoondar, J., Phelps, C., Vaught, T., Ball, S., LeRoith, T., Monahan, J., Chen, S., Dandro, A., Boone, J., Jobst, P., Vance, A., Wertz, N., Bergman, Z., Sun, X., Polejaeva, I., Butlet, J., Dai, Y., Ayares, D., Wells, K., (2011). Generation of antibody- and B cell-deficient pigs by targeted disruption of the J-region gene segment of the heavy chain locus. Transgenic Research, 20:3, 625-641.
      • Gregg, K., Xiang, T., Arenivas, S., Hwang, E., Arenivas, F., Chen, S., Walker, S., Picou, A., Polejaeva, I., (2011). Risk assessment of porcine reproductive and Respiratory syndrome virus (PRRSV) transmission via somatic cell nuclear transfer (SCNT) embryo production using oocytes from commercial abattoirs. . Anim Reprod Sci, 125:1-4, 148-157.
      • Ramsoondar, J., Mendicino, M., Phelps, C., Vaught, T., Ball, S., Monahan, J., Chen, S., Dandro, A., Boone, J., Jobst, P., Vance, A., Wertz, N., Polejaeva, I., Butler, J., Dai, Y., Ayares, D., Wells, K., (2011). Targeted disruption of the porcine immunoglobulin kappa light chain locus. Transgenic Research, 20:3, 643-653.
      • Gregg, K., Gosch, G., Guerra, T., Chen, S., Xiang, T., Broek, D., Bruner, B., Polejaeva, I., (2010). Large scale in vivo risk assessment of bovine viral diarrhea virus (BVDV) transmission through transfer of bovine embryos produced via somatic cell nuclear transfer (SCNT).. Theriogenology, 74:7, 1264-70.
      • Gregg, K., Riddell, K., Chen, S., Galik, P., Xiang, T., Guerra, T., Marley, M., Polejaeva, I., Givens, M., (2010). Risk and prevention of bovine viral diarrhea virus (BVDV) transmission through embryo production via somatic cell nuclear transfer (SCNT) using oocytes from persistently infected donors. . Theriogenology, 74:1, 1-10.
      • Gregg, K., Chen, S., Guerra, T., Sadeghieh, S., Xing, T., Meredith, J., Polejaeva, I., (2009). A sensitive and efficient detection method for bovine viral diarrhea virus (BVDV) in single preimplantation bovine embryos.. Theriogenology, 71, 966-974.
      • Gregg, K., Chen, S., Sadeghieh, S., Guerra, T., Xiang, T., Meredith, J., Polejaeva, I., (2009). Experimental risk assessment of bovine viral diarrhea virus transmission via in vitro embryo production using somatic cell nucleus transfer.. Theriogenology, 72:1, 99-110.
      • Gregg, K., Polejaeva, I., (2009). Risk of equine infectious anemia virus disease transmission through in vitro embryo production using somatic cell nucleus transfer. Theriogenology, 72:3, 289-299.
      • Zhou, W., Sadeghieh, S., Abruzzese, R., Uppada, S., Meredith, J., Ohlrichs, C., Broek, D., Polejaeva, I., (2009). Transcript levels of several epigenome regulatory genes in bovine somatic donor cells are not correlated with their cloning efficiency. . Cloning and Stem Cells, 11, 397-405.
      • Zhou, W., Xiang, T., Walker, S., Abruzzese, R., Hwang, E., Farrar, V., Findeisen, B., Sadeghieh, S., Arenivas, F., Chen, S., Polejaeva, I., (2008). Aggregation of Bovine Cloned Embryos at the Four –Cell Stage Stimulated Gene Expression and In Vitro Embryo Development. Molecular Reproduction and Development, 75, 1281-1289.
      • Zhou, W., Xiang, T., Walker, S., Farrar, V., Hwang, E., Findeisen, B., Sadeghieh, S., Arenivas, F., Abruzzese, R., Polejaeva, I., (2008). Global Gene Expression Analysis of Bovine Blastocysts Produced by Multiple Methods. Molecular Reproduction and Development, 75, 744-758.
      • Walker, S., Christenson, R., Ruiz, R., Reeves, D., Pratt, S., Arenivas, F., Williams, N., Bruner, B., Polejaeva, I., (2007). Comparison of meat composition from offspring of cloned and conventionally produced boars. Theriogenology, 67, 178-184.
      • Williams, N., Walker, S., Reeves, D., Sherrer, E., Galvin, J., Polejaeva, I., Rampacek, G., Benshek, L., Christenson, R., Graves, W., Pratt, S., (2006). A Comparison of Reproductive Characteristics of Boars Generated by Somatic Cell Nuclear Transfer to Highly Related Conventionally Produced Boars. Cloning and Stem Cells, 8, 130-139.
      • Ji, W., Zhou, W., Abruzzese, R., Guo, W., Blake, A., Davis, S., Davis, S., Polejaeva, I., (2005). A method of determining zygosity of transgenic zebra fish by TaqMan real-time PCR. Analytical Biochem, 344:2, 240-246.
      • Zhou, W., Abruzzese, R., Polejaeva, I., Davis, S., Davis, S., Ji, W., (2005). Amplification of nanogram amount of total RNA by the SMART-based PCR method for high-density oligonucleotide microarrays.. Clinical Chemistry, 51:12, 2354-2356.
      • Gwazdauskas, F., Walters, A., McGilliard, M., Ball, S., Ellefson, N., Flesher, S., Keyes, L., Nicholson, W., Rosoff, K., Strahsmeier, K., Wheeler, M., Polejaeva, I., Ayares, D., (2003). Behavioral comparisons of cloned and non-cloned pigs. Journal of Animal and Veterinary Advances, 2:8, 430-436.
      • Phelps, C., Koike, C., Vaught, T., Boone, J., Wells, K., Chen, S., Ball, S., Specht, S., Monahan, J., Jobst, P., Sharma, S., Lamborn, A., Polejaeva, I., Moore, M., Demetris, A., Rudert, W., Bottino, R., Bertera, S., Trucco, M., Starzl, T., Dai, Y., Ayares, D., (2003). Production of a1,3-galactosyltransferase deficient pigs. Science, 299, 411-414.
      • Chen, S., Vaught, T., Monahan, J., Boone, J., Emslie, E., Jobst, P., Lamborn, A., Schnieke, A., Robertson, L., Colman, A., Dai, Y., Polejaeva, I., Ayares, D., (2002). Efficient production of transgenic cloned calves using preimplantation screening. Biology of Reproduction, 67, 1488-1472.
      • Dai, Y., Vaught, T., Boone, J., Chen, S., Phelps, C., Ball, S., Monahan, J., Jobst, J., McCreath, K., Lamborn, A., Cowell-Lucero, J., Wells, K., Colman, A., Polejaeva, I., Ayares, D., (2002). Targeted disruption of the α1, 3-galactosyltransferase gene in cloned pigs. Nature biotechnology, 20, 251-255.
      • Polejaeva, I., (2001). Pig cloning: advances and applications. Reproduction, 58, 293-300.
      • Polejaeva, I., Chen, S., Vaught, T., Page, R., Mullins, J., Dai, Y., Boone, J., Walker, S., Ayares, D., Colman, A., Campbell, K., (2000). Cloned Piglets Produced by Nuclear Transfer from Adult Somatic Cells. Nature, 407, 86-90.
      • Polejaeva, I., Campbell, K., (2000). New advances in somatic cell nuclear transfer: application in transgenesis. Theriogenology, 53, 117-126.
      • Polejaeva, I., Reed, W., Bunch, T., Ellis, L., White, K., (1997). Effect of Prolactin on the Reactivation of Mink blastocysts in Obligate Diapause in Vitro and Embryonic Stem Cells Establishment. Journal of Reproduction & Fertility, 109, 229-236.
      • Wolf, E., Kramer, R., Polejaeva, I., Thoenen, H., Brem, G., (1994). Efficient generation of chimaeric mice using embryonic stem cells after long-term culture in the presence of ciliary neurotrophic factor. Transgenic Research, 3, 152-158.

      * Has not been peer reviewed

      Publications - Literary Journal

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        Publications - MultiMedia

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          Publications - Technical Reports

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            Publications - Translations & Transcripts

              Publications - Other

                Newsletter

              • Polejaeva, I., Gregg, K., Kraemer, D., (2011). Biosafety issues and mitigations regarding the use of tissues and oocytes for cloning. Embryo Transfer Newsletter
              • Other

              • Polejaeva, I., Wang, Z., Hu, S., Ranjan, R., Davies, C., Thomas, A., White, K.L, (2015). Method for transgenic bovidae expressing cardiac fibrosis and associated pathology. United States Patent Application Publication *

              * Has not been peer reviewed

              Graduate Students Mentored

              Jacob Keim, Animal, Dairy and Veterinary Sciences, January 2017
              Iuri Perisse, Animal, Dairy and Veterinary Sciences, June 2016
              Misha Regouski, Animal, Dairy and Veterinary Sciences, January 2015
              Min Yang, Animal, Dairy and Veterinary Sciences, September 2012
              Kirsten Gash, Animal, Dairy and Veterinary Sciences, September 2012 - December 2015