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Zhongde Wang

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Animal, Dairy and Veterinary Sciences

Transgenics, Molecular Biology, Stem Cell Research


Contact Information

Go toOffice Location: USTAR room 305k
DialPhone: 435-797-9668

Educational Background

Nuclear reprogramming by somatic cell nuclear transfer, (Embryonic stem cells and genetic engineering), Massachusetts Institute of Technology, 2004
PhD, Molecular and Cellular Biology, University of Massachusetts at Amherst, 2000
Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue


Genome Engineering, Epigenetics and Stem Cells // Program goals: Developing novel genome engineering tools for improving livestock (e.g. Mastitis resistance) and for creating transgenic animal models for human diseases; Establish embryonic stem cells from livestock species and from hamsters.


Dr. Zhongde Wang is a professor in the Department of Animal, Dairy and Veterinary Sciences at Utah State University. In addition to his academic appointment, Dr. Wang is also the President and Chief Scientific Officer and co-founder of Auratus Bio, a biotech company specialized in animal genetic engineering. Dr. Wang received his Ph.D. degree in molecular and cellular biology from University of Massachusetts at Amherst in 2000 and did his postdoctoral training at the Whitehead Institute/Massachusetts Institute of Technology.

Teaching Interests

Metabolic syndromes, biotechnology, and stem cells

Research Interests

Dr. Wang’s research team employs modern genome engineering tools (such as CRISPR/Cas9) and assisted reproduction technologies (such as IVF, ICSI, and somatic cell nuclear transfer or cloning) to create genetically engineered animal models of human diseases. The Wang laboratory is the first to succeed in developing gene targeting tools in the golden Syrian hamster. His laboratory is also actively pursuing a better understanding of the epigenetics of stem cells. Current projects in Wang’s laboratory include genetic engineering in golden Syrian hamsters to create animal models of human disease. His laboratory is also actively engaging in research activities in the development of genetically engineered goats and sheep as animal models of cardiovascular diseases, lung cancer, and cystic fibrosis. The Wang laboratory is also studying embryonic stem (ES) cells and induced pluripotent stem (iPS) cells from important farm animal species. Another research focus in the Wang laboratory is by employing immunological and genetic approaches to understand and improve mastitis resistance in dairy cattle. We are always looking for highly motivated, ambitious, creative, and hardworking individuals to join the lab for postdoctoral and MS/PhD trainings. If you think you have these qualities, please contact Dr. Wang directly at


CAAS Faculty Researcher of the Year, 2017

ADVS Faculty Researcher of the Year Award, 2017

Lalor Foundation Postdoctoral Fellowships , 2003

Lalor Foundation

Lalor Foundation Postdoctoral Fellowships , 2002

Lalor Foundation

Publications - Abstracts

Publications - Books & Book Chapters

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Publications - Fact Sheets

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    Publications - Curriculum

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      Publications - Journal Articles

        Academic Journal

      • Hickerson, B.T, Westover, J.B, Wang, Z., Lee, Y., Gowen, B., (2019). Guinea pig transferrin receptor 1 mediates cellular entry of Junín virus and other pathogenic New World arenaviruses. J Virol
      • Kurz, J.P, Richards, M.P, Garcia, M., Wang, Z., (2019). Exogenous phospholipase A2affects inflammatory gene expression in primary bovine mammary epithelial cells. Journal of Dairy Research, doi: 10.1017/S0022029919000232
      • Wu, H., Fan, Z., Brandsrud, M., Meng, Q., Bobbitt, M., Regouski, M., Stott, R.D, Sweat, A., Crabtree, J., Hogan, R.J, Tripp, R.A, Wang, Z., Polejaeva, I., Sullivan, E.J, (2019). Generation of H7N9-specific human polyclonal antibodies from a transchromosomic goat (caprine) system.. Scientific reports, 9:1, 366.
      • Siddharthan, V., Miao, J., Van Wettere, A., Li , R., Wu, H., Sullivan, E., Jiao, J., Hooper, J.W, Safronetz, D., Morrey, J.D, Julander, J., Wang, Z., (2019). Human Polyclonal Antibodies Produced from Transchromosomal Bovine Provides Prophylactic and Therapeutic Protections Against Zika Virus Infection in <i>STAT2</i> KO Syrian Hamsters.. Viruses, 11:2
      • Li , R., Miao, J., Wang, Z., (2019). Production of Genetically Engineered Porcine Embryos by Handmade Cloning.. Methods in molecular biology (Clifton, N.J.), 1874, 347-360.
      • Zou, J., Li , R., Wang, Z., Yang, J., (2019). Studies of the Periciliary Membrane Complex in the Syrian Hamster Photoreceptor.. Advances in experimental medicine and biology, 1185, 543-547.
      • Atkins, C., Miao, J., Kalveram, B., Juelich, T., Smith, J.K, Perez, D., Zhang, L., Westover JLB, , Van Wettere, A., Gowen, B., Wang, Z., Freiberg, A.N, (2018). Natural History and Pathogenesis of Wild-Type Marburg Virus Infection in STAT2 Knockout Hamsters.. The Journal of infectious diseases, 218:suppl_5, S438-S447.
      • Hickerson, B.T, Westover, J.B, Van Wettere, A., Rigas, J., Miao, J., Conrad, B.L, Motter, N.E, Wang, Z., Gowen, B., (2018). Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters.. Viruses, 10:11
      • Jangra, R.K, Herbert, A.S, Li , R., Jae, L.T, Kleinfelter, L.M, Slough, M.M, Barker, S.L, Guardado-Calvo, P., Román-Sosa, G., Dieterle, M.E, Kuehne, A.I, Muena, N.A, Wirchnianski, A.S, Nyakatura, E.K, Fels, J.M, Ng, M., Mittler, E., Pan, J., Bharrhan, S., Wec, A.Z, Lai, J.R, Sidhu, S.S, Tischler, N.D, Rey, F.A, Moffat, J., Brummelkamp, T.R, Wang, Z., Dye, J.M, Chandran, K., (2018). Protocadherin-1 is essential for cell entry by New World hantaviruses.. Nature, 563:7732, 559-563.
      • Li , R., Miao, J., Tabaran, A.F, O'Sullivan, M.G, Anderson, K.J, Scott, P., Wang, Z., Cormier, R.T, (2018). A novel cancer syndrome caused by <i>KCNQ1</i>-deficiency in the golden Syrian hamster.. Journal of carcinogenesis, 17, 6.
      • Fan, Z., Perisse, I.V, Cotton, C.U, Regouski, M., Meng, Q., Domb, C., Van Wettere, A., Wang, Z., Harris, A., White, K.L, Polejaeva, I., (2018). A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene.. JCI insight, 3:19
      • Kurz, J., Zhou, Y., Weiss, R., Wilson, D., Rood, K., Liu, G., Wang, Z., (2018). A genome-wide association study for mastitis resistance in phenotypically well-characterized Holstein dairy cattle using a selective genotyping approach. BMC Genomics
      • Miao, J., Ying, B., Li , R., Tollefson, A.E, Spencer, J.F, Wold WSM, , Song, S.H, Kong, I.K, Toth, K., Wang, Y., Wang, Z., (2018). Characterization of an N-Terminal Non-Core Domain of RAG1 Gene Disrupted Syrian Hamster Model Generated by CRISPR Cas9.. Viruses, 10:5
      • Zhou, G., Wei, H., Wang, X., Yang, M., Bunch, T.D, Polejaeva, I., White, K.L, Wang, Z., Meng, Q., (2018). Serial Culture Is Critical for In Vitro Development of Parthenogenetic Embryos in the Golden Syrian Hamster.. Cellular reprogramming
      • Li , R., Miao, J., Fan, Z., Song, S., Kong, I.K, Wang, Y., Wang, Z., (2018). Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex.. Journal of visualized experiments : JoVE131
      • Westover, J.B, Rigas, j., Van Wettere, A., Li , R., Hickerson, B.T, Jung, K.H, Miao, J., Reynolds, E.S, Conrad, B.L, Nielson, S., Furuta, Y., Thangamani, S., Wang, Z., Gowen, B., (2017). Heartland virus infection in hamsters deficient in type I interferon signaling: Protracted disease course ameliorated by favipiravir. Virology, 511, 175-183.
      • Stein, D.R, Golden, J.W, Griffin, B.D, Warner, B.M, Ranadheera, C., Scharikow, L., Sloan, A., Frost, K.L, Kobasa, D., Booth, S.A, Josleyn, M., Ballantyne, J., Sullivan, E., Jiao, J.A, Wu, H., Wang, Z., Hooper, J.W, Safronetz, D., (2017). Human polyclonal antibodies produced in transchromosomal cattle prevent lethal Zika virus infection and testicular atrophy in mice.. Antiviral research, 146, 164-173.
      • Siddharthan, V., Van Wettere, A., Li , R., Miao, J., Wang, Z., Morrey, J.D, Julander, J., (2017). Zika virus infection of adult and fetal STAT2 knock-out hamsters.. Virology, 507, 89-95.
      • Gowen, B., Westover, J.B, Miao, J., Van Wettere, A., Rigas, J., Hickerson, B.T, Jung, K.H, Li, R., Conrad, B.L, Nielson, S., Furuta, Y., Wang, Z., (2017). Modeling severe fever with thrombocytopenia syndrome virus infection in golden Syrian hamsters: Importance of STAT2 in preventing disease and effective treatment with favipiravir. Journal of Virology
      • Polejaeva, I., Ranjan, R., Davies, C., Regouski, M., Hall, J., Olsen, A.L, Meng, Q., Rutigliano, H., Dosdall, D., Angel, N., Sachse, F., Seidel, T., Thomas, A., Stott, R.D, Panter, K., Lee, P., Van Wettere, A., Stevens, J.R, Wang, Z., MacLeod, R., Marrouche, N., White, K.L, (2016). Increased Susceptibility to Atrial Fibrillation Secondary to Atrial Fibrosis in Transgenic Goats Expressing Transforming Growth Factor-β1. Journal of Cardiovascular Electrophysiology. , 27:10, 1220-1229.
      • Fan, Z., Meng, Q., Bunch, T.D, White, K.L, Wang, Z., (2016). Effective cryopreservation of golden Syrian hamster embryos by open pulled straw vitrification.. Laboratory animals, 50:1, 45-53.
      • Toth, K., Lee, S.R, Ying, B., Spencer, J.F, Tollefson, A.E, Sagartz, J.E, Kong, I.K, Wang, Z., Wold, W.S, (2015). STAT2 Knockout Syrian Hamsters Support Enhanced Replication and Pathogenicity of Human Adenovirus, Revealing an Important Role of Type I Interferon Response in Viral Control.. PLoS pathogens, 11:8, e1005084.
      • Fakruzzaman, M., Ghanem, N., Bang, J.I, Ha, A.N, Wang, Z., Lee, D.S, Kong, I.K, (2015). Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos.. Animal reproduction science, 159, 172-83.
      • Matsushita, H., Sano, A., Wu, H., Wang, Z., Jiao, J.A, Kasinathan, P., Sullivan, E.J, Kuroiwa, Y., (2015). Species-Specific Chromosome Engineering Greatly Improves Fully Human Polyclonal Antibody Production Profile in Cattle.. PloS one, 10:6, e0130699.
      • Ha, A.N, Fakruzzaman, M., Bang, J.I, Deb, G.K, Wang, Z., Kong, I.K, (2015). Effects of co-culture of cumulus oocyte complexes with denuded oocytes during in vitro maturation on the developmental competence of cloned bovine embryos.. Reproduction in domestic animals, 50:2, 292-8.
      • Wang, Z., (2015). Genome engineering in cattle: recent technological advancements.. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 23:1, 17-29.
      • Fan, Z., Li, W., Lee, S., Meng, Q., Bunch, T., White, K., Kong, I., Wang, Z., (2014). Efficient gene targeting in golden Syrian hamsters by the CRISPR/Cas9 system. PLOS ONE, doi: 10.1016/j.cryobiol.2013.11.007
      • Kim, S., Bang, J., Fakruzzaman , M., Lee, K., Ko, D., Ghanem, N., Wang, Z., Kong, I., (2014). Effects of Flunixin Meglumin and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos in vitro. Reproduction in Domestic Animals, doi: 10.1016/j.cryobiol.2013.11.007
      • Gong, J., Wang, Z., Polejaeva, I., Salgia, R., Kao, C., Chen, C., Chen, L., (2014). Activating the expression of human K-rasG12D stimulates oncogenic transformation in transgenic goat fetal fibroblast cells. PLoS ONE
      • Matsushita, H., Sano, A., Wu, H., Jiao, J., Kasinathan, P., Sullivan, E.J, Wang, Z., Kuroiwa, Y., (2014). Triple Immunoglobulin Gene Knockout Transchromosomic (Tc) Cattle: Bovine Lambda Cluster Deletion And Its Effect On Fully Human Polyclonal Antibody Production. PLoS ONE
      • Ha, A., Lee, S., Jeong-Seon, J., Han-Seul, P., Sang-HO, L., Jong-In, J., Sessions, B., Wang, Z., White, K.L, Kong, I., (2013). Development of a modified straw method for vitrification of in vitro-produced bovine blastocysts and various genes expression in between the methods. Cryobiology, doi: 10.1016/j.cryobiol.2013.11.007
      • Jo, H., Bang, J., Kim, S., Choi, B., Jin, J., Kim, H., Jung, I., Suh, ., Ghanem, N., Wang, Z., Kong, I., (2013). Production of female bovine embryos with sex-sorted sperm using intracytoplasmic sperm injection: Efficiency and in vitro developmental competence. Theriogenology, doi: doi:10.1016/j.theriogenology.2013.11.010
      • Kim, S., Bang, J., Fakruzzaman, M., Lee, K., Jin, J., Ko, D., Ghanem, N., Wang, Z., Kong, I., Effect of flunixin meglumin and prostaglandin F2α treatments on the development and quality of bovine embryos in vitro. Theriogenology/Elsevier
      • Sano, A., Matsushita, H., Wu, H., Jiao, J., Kasinathan, P., Sullivan, E.J, Wang, Z., Kuroiwa, Y., (2013). Physiological level production of antigen-specific human immunoglobulin in cloned transchromosomic cattle. PLoS ONE, 8:10, e78119. doi: doi:10.1371/journal.pone.0078119
      • Mesquita, F., Machado, S., Drnevich , J., Borowicz, P., Wang, Z., Nowak, R., (2013). Influence of cloning by chromatin transfer on placental gene expression at day 45 of pregnancy in cattle. Animal Reproduction Science, 136:4, 231-44. doi: 10.1016/j.anireprosci.2012.10.030
      • Wang, J., Liu, Y., Li , Z., Wang, Z., Tan, L.X, Ryu, M.J, Meline, B., Du , J., Young, K.H, Ranheim, E., Chang, Q., Zhang, J., (2011). Endogenous oncogenic Nras mutation initiates hematopoietic malignancies in a dose- and cell type-dependent manner.. Blood, 118:2, 368-79.
      • Liu, G.E, Hou, Y., Robl, J.M, Kuroiwa, Y., Wang, Z., (2011). Assessment of genome integrity with array CGH in cattle transgenic cell lines produced by homologous recombination and somatic cell cloning.. Genome integrity, 2:1, 6.
      • Rodriguez-Sosa, J.R, Rathi, R., Wang, Z., Dobrinski, I., (2011). Development of bovine fetal testis tissue after ectopic xenografting in mice.. Journal of andrology, 32:3, 271-81.
      • Wang, Z., (2011). Cloning mice and ES cells by nuclear transfer from somatic stem cells and fully differentiated cells.. Methods in molecular biology (Clifton, N.J.), 770, 551-69.
      • Wang, Z., (2011). Derivation of mouse embryonic stem cell lines from blastocysts produced by fertilization and somatic cell nuclear transfer.. Methods in molecular biology (Clifton, N.J.), 770, 529-49.
      • McLean, C.A, Wang, Z., Babu, K., Edwards, A., Kasinathan, P., Robl, J., Sheppard, A.M, (2010). Normal development following chromatin transfer correlates with donor cell initial epigenetic state.. Animal reproduction science, 118:2-4, 388-93.
      • Rodriguez-Osorio, N., Wang, Z., Kasinathan, P., Page, G.P, Robl, J.M, Memili, E., (2009). Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos.. BMC genomics, 10, 190.
      • Robl, J.M, Wang, Z., Kasinathan, P., Kuroiwa, Y., (2007). Transgenic animal production and animal biotechnology.. Theriogenology, 67:1, 127-33.
      • Blelloch, R., Wang, Z., Meissner, A., Pollard, S., Smith, A., Jaenisch, R., (2006). Reprogramming efficiency following somatic cell nuclear transfer is influenced by the differentiation and methylation state of the donor nucleus.. Stem cells (Dayton, Ohio), 24:9, 2007-13.
      • Wang, Z., Jaenisch, R., (2004). At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation.. Developmental biology, 275:1, 192-201.
      • Kasinathan, P., Knott, J.G, Wang, Z., Jerry, D.J, Robl, J.M, (2001). Production of calves from G1 fibroblasts.. Nature biotechnology, 19:12, 1176-8.
      • Hansen, D.E, Roberts, R.A, Wang, Z., Sgasawara, R.J, (2001). RJS8-1, Mouse Monoclonal to FK-520 (Anti-Ascomycin). Hybridoma, 20, 207-208.
      • Wang, Z., Raifu, M., Howard, M., Smith, L., Hansen, D.R, Goldsby, R., Ratner, D., (2000). Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3' to 5' exonuclease activity.. Journal of immunological methods, 233:1-2, 167-77.
      • Pinkham, J.L, Wang, Z., Alsina, J., (1997). Heme regulates SOD2 transcription by activation and repression in Saccharomyces cerevisiae.. Current genetics, 31:4, 281-91.

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      Publications - Literary Journal

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        Publications - MultiMedia

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          Publications - Technical Reports

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            Publications - Translations & Transcripts

              Publications - Other


              • Wang, Z., (2016). Correction: STAT2 Knockout Syrian Hamsters Support Enhanced Replication and Pathogenicity of Human Adenovirus, Revealing an Important Role of Type I Interferon Response in Viral Control.. PLoS pathogens
              • Polejaeva, I., Wang, Z., Hu, S., Ranjan, R., Davies, C., Thomas, A., White, K.L, (2015). Method for transgenic bovidae expressing cardiac fibrosis and associated pathology. United States Patent Application Publication *

              * Has not been peer reviewed

              Graduate Students Mentored

              Joseph Howes, Animal, Dairy and Veterinary Sciences, September 2017
              Nick Robl, Animal, Dairy and Veterinary Sciences, September 2016
              Jinxin Miao, Other, September 2015
              Dane Rasmussen, Animal, Dairy and Veterinary Sciences, September 2013
              Jacqueline LaRose, Animal, Dairy and Veterinary Sciences, September 2012